Conventional Versus Molecular Methods for Diagnosis of Burkholderia cepacia from Different Clinical Samples of Iraqi Patients
The study aims at isolating and identification of Burkholderia cepacia bacteria from clinical samples from various pathological conditions such as diabetic foot ulcers, burn, wound, sputum and urine. The present study includes 280 samples collected from patients suffering from diabetic foot ulcer, cystic fibrosis, burns, sputum, and wounds who attend Alsader Medical City and Al-Hakim General Hospital during the period extended from September 2020 to February 2021 ( men and women ) with age groups between (1 -75) years. The identification of bacterial isolates were detected by classical and molecular technique (PCR), where the frequency among males 213 (79.2%) was more than that in female 67 (20.8%). The samples distribution is made according to age group; it appears high for the following high 26.8% with group (31-45) years male and female; 22% with group (31-45) years in male and 7.6% with the group (1-15) years in female. The results revealed that 42/80 specimens of the total number of samples are Burkholderia cepacia by using 16SrRNA gene to differentiate the B. cepacia from other (G-) bacteria, and 30/80 recA gene to differentiated B. cepacia from B. cepacia complex.