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Volume 1, Issue 3 (July 2022)
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Estimation of Botanical Diversity by Molecular Marker Methods
Zahraa A. N. Al-Yassiry
 Department of Biology, College of Science, University of Babylon - Iraq
 ayaziabdalla2014@gmail.com
 Corresponding Author
Basheer Al-Alwani
 Department of Biology, College of Science, University of Babylon - Iraq
ISSN(e): 2790-296X
ISSN(p): 2957-5826
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Prof. Dr. Emad Al-Mulla
 
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Various molecular methods could be utilized in order to investigate botanical diversity. Arbitrary primed DNA, variable number of tandem repeats (VNTR), Restriction fragment length polymorphism (RFLP), polymerase chain reaction (PCR) sequencing, amplified fragment length polymorphism (AFLP), and sequence-tagged simple sequence repeats (SSRs) are all briefly reviewed. DNA-based approaches have recently been proved to be useful for crucial tasks, like specimen identification and targeted screening for expected or known invaders, according to a recent study. Prior to more ambitious applications, as extensive surveys of complex environmental samples and propagule pressure prediction, could be conducted, considerable technological obstacles should be solved. The aim of the current review was to estimate the molecular techniques used for assessing the genetic diversity of plants. The degree of variation among the plant species based on genetics is described as the genetic diversity of plants, evaluating the possible value regarding the current invasive species monitoring methods.

  1. Agarwal, M., Shrivastava, N., & Padh, H. (2008). Advances in molecular marker techniques and their applications in plant sciences. Plant Cell Reports, 27(4), 617-631. https://doi.org/10.1007/s00299-008-0507-z
  2. Al- Al-Qurainy, F., Khan, S., Tarroum, M., Al-Hemaid, F. M., & Ali, M. A. (2011). Molecular authentication of the medicinal herb Ruta graveolens (Rutaceae) and an adulterant using nuclear and chloroplast DNA markers. Genetics and Molecular Research, 10(4), 2806-16.
  3. Al Khateeb, W., Bahar, E., Lahham, J., Schroeder, D., & Hussein, E. (2013). Regeneration and assessment of genetic fidelity of the endangered tree Moringa peregrina (Forsk.) Fiori using Inter Simple Sequence Repeat (ISSR). Physiology and Molecular Biology of Plants, 19(1), 157-164. https://doi.org/10.1007/s12298-012-0149-z
  4. Al‐Mahmoud, M. E., Al‐Dous, E. K., Al‐Azwani, E. K., & Malek, J. A. (2012). DNA‐based assays to distinguish date palm (Arecaceae) gender. American Journal of Botany, 99(1), e7-e10. https://doi.org/10.3732/ajb.1100425
  5. Al-Qurainy, F., Khan, S., Al-Hemaid, F. M., Ali, M. A., Tarroum, M., & Ashraf, M. (2011). Assessing molecular signature for some potential date (Phoenix dactylifera L.) cultivars from Saudi Arabia, based on chloroplast DNA sequences rpoB and psbA-trnH. International Journal of Molecular Sciences, 12(10), 6871-6880. https://doi.org/10.3390/ijms12106871
  6. Al-Qurainy, F., Khan, S., Ali, M. A., Al-Hemaid, F. M., Tarroum, M., & Ashraf, M. (2011). Authentication of Ruta graveolens and its adulterant using internal transcribed spacer (ITS) sequences of nuclear ribosomal DNA. Pakistan Journal of Botany, 43(3), 1613-1620.
  7. Alwhibi, M. S. (2017). Characterization of seed storage protein patterns of Heliotropium digynum. Saudi Journal of Biological Sciences, 24(6), 1404-1409. https://doi.org/10.1016/j.sjbs.2016.08.008
  8. Bafeel, S. O., Arif, I. A., Bakir, M. A., Al Homaidan, A. A., Al Farhan, A. H., & Khan, H. A. (2012). DNA barcoding of arid wild plants using rbcL gene sequences. Genet. Mol. Res, 11(3), 1934-41. http://dx.doi.org/10.4238/2012.July.19.12
  9. Bafeel, S. O., Arif, I. A., Bakir, M. A., Khan, H. A., Al Farhan, A. H., Al Homaidan, A. A., ... & Thomas, J. (2011). Comparative evaluation of PCR success with universal primers of maturase K (matK) and ribulose-1, 5-bisphosphate carboxylase oxygenase large subunit (rbcL) for barcoding of some arid plants. Plant Omics, 4(4), 195-198.
  10. Basahi, M. (2015). ISSR-based analysis of genetic diversity among sorghum landraces growing in some parts of Saudi Arabia and Yemen. Comptes Rendus Biologies, 338(11), 723-727. https://doi.org/10.1016/j.crvi.2015.09.003
  11. Besansky, N. J., Severson, D. W., & Ferdig, M. T. (2003). DNA barcoding of parasites and invertebrate disease vectors: what you don't know can hurt you. Trends in Parasitology, 19(12), 545-546. https://doi.org/10.1016/j.pt.2003.09.015
  12. Bhagyawant, S. S. (2016). RAPD-SCAR markers: an interface tool for authentication of traits. Journal of Biosciences and Medicines, 4(01), 1-9. http://dx.doi.org/10.4236/jbm.2016.41001
  13. Bhandari, H. R., Bhanu, A. N., Srivastava, K., Singh, M. N., & Shreya, H. A. (2017). Assessment of genetic diversity in crop plants-an overview. Advances in Plants & Agriculture Research, 7(3), 279-286. https://doi.org/10.15406/apar.2017.07.00255
  14. Botstein, D., White, R. L., Skolnick, M., & Davis, R. W. (1980). Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American journal of human genetics, 32(3), 314. https://pubmed.ncbi.nlm.nih.gov/6247908
  15. Braslavsky, I., Hebert, B., Kartalov, E., & Quake, S. R. (2003). Sequence information can be obtained from single DNA molecules. Proceedings of the National Academy of Sciences, 100(7), 3960-3964. https://doi.org/10.1073/pnas.0230489100
  16. Caesar, R. M., Sörensson, M., & Cognato, A. I. (2006). Integrating DNA data and traditional taxonomy to streamline biodiversity assessment: an example from edaphic beetles in the Klamath ecoregion, California, USA. Diversity and Distributions, 12(5), 483-489. https://doi.org/10.1111/j.1366-9516.2006.00237.x
  17. Clarke, J., Wu, H. C., Jayasinghe, L., Patel, A., Reid, S., & Bayley, H. (2009). Continuous base identification for single-molecule nanopore DNA sequencing. Nature nanotechnology, 4(4), 265-270. https://doi.org/10.1038/nnano.2009.12
  18. El Rabey, H. A., Al-Malki, A. L., Abulnaja, K. O., Ebrahim, M. K., Kumosani, T., & Khan, J. A. (2014). Phylogeny of ten species of the genus Hordeum L. as revealed by AFLP markers and seed storage protein electrophoresis. Molecular Biology Reports, 41(1), 365-372. https://doi.org/10.1007/s11033-013-2870-2
  19. Fang, Y., Wu, H., Zhang, T., Yang, M., Yin, Y., Pan, L., ... & Yu, J. (2012). A complete sequence and transcriptomic analyses of date palm (Phoenix dactylifera L.) mitochondrial genome. PloS one, 7(5), e37164. https://doi.org/10.1371/journal.pone.0037164
  20. Foster, J. T., Allan, G. J., Chan, A. P., Rabinowicz, P. D., Ravel, J., Jackson, P. J., & Keim, P. (2010). Single nucleotide polymorphisms for assessing genetic diversity in castor bean (Ricinus communis). BMC Plant Biology, 10(1), 1-11. https://doi.org/10.1186/1471-2229-10-13
  21. Fu, Y. B. (2015). Understanding crop genetic diversity under modern plant breeding. Theoretical and Applied Genetics, 128(11), 2131-2142. https://doi.org/10.1007/s00122-015-2585-y
  22. Hajrah, N. H., Obaid, A. Y., Atef, A., Ramadan, A. M., Arasappan, D., Nelson, C. A., ... & Jansen, R. K. (2017). Transcriptomic analysis of salt stress responsive genes in Rhazya stricta. PloS one, 12(5), e0177589. https://doi.org/10.1371/journal.pone.0177589
  23. Joshi, S. P., Ranjekar, P. K., & Gupta, V. S. (1999). Molecular markers in plant genome analysis. Current Science, 230-240. https://www.jstor.org/stable/24102526
  24. Karp, A. (1997). Molecular tools in plant genetic resources conservation: a guide to the technologies (No. 2). Bioversity International.
  25. Karp, A., Seberg, O. L. E., & Buiatti, M. (1996). Molecular techniques in the assessment of botanical diversity. Annals of Botany, 78(2), 143-149. https://doi.org/10.1006/anbo.1996.0106
  26. Kumar, P., Gupta, V. K., Misra, A. K., Modi, D. R., & Pandey, B. K. (2009). Potential of molecular markers in plant biotechnology. Plant Omics, 2(4), 141-162.
  27. Lerner, H. R., & Fleischer, R. C. (2010). Prospects for the use of next-generation sequencing methods in ornithology. The Auk, 127(1), 4-15. https://doi.org/10.1525/auk.2010.127.1.4
  28. Lynch, M., & Walsh, B. (1998). Genetics and analysis of quantitative traits (Vol. 1, pp. 535-557). Sunderland, MA: Sinauer.
  29. Margulies, M., Egholm, M., Altman, W. E., Attiya, S., Bader, J. S., Bemben, L. A., ... & Rothberg, J. M. (2005). Genome sequencing in microfabricated high-density picolitre reactors. Nature, 437(7057), 376-380. https://doi.org/10.1038/nature03959
  30. Mishra, K. K., Fougat, R. S., Ballani, A., Vinita, T., Yachana, J., and Madhumati, B. (2014). Potential and application of molecular markers techniques for plant genome analysis. International Journal of Pure & Applied Bioscience, 2(1), 169-188.
  31. Morris, A. B., & Shaw, J. (2018). Markers in time and space: a review of the last decade of plant phylogeographic approaches. Molecular Ecology, 27(10), 2317-2333. https://doi.org/10.1111/mec.14695
  32. Mosa, K. A., Ismail, A., & Helmy, M. (2017). Introduction to plant stresses. In Plant stress tolerance (pp. 1-19). Springer, Cham. https://doi.org/10.1007/978-3-319-59379-1_1
  33. Ozsolak, F., Platt, A. R., Jones, D. R., Reifenberger, J. G., Sass, L. E., McInerney, P., ... & Milos, P. M. (2009). Direct RNA sequencing. Nature, 461(7265), 814-818. https://doi.org/10.1038/nature08390
  34. Reddy, M. P., Sarla, N., & Siddiq, E. A. (2002). Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding. Euphytica, 128, 9-17. https://doi.org/10.1023/A:1020691618797
  35. Ridout, C. J., Donini, P., Ridout, C. J., & Donini, P. (1999). Use of AFLP in cereals research. Trends in Plant Science, 4(2), 76-79. https://doi.org/10.1016/S1360-1385(98)01363-6
  36. Ronaghi, M., Karamohamed, S., Pettersson, B., Uhlén, M., & Nyrén, P. (1996). Real-time DNA sequencing using detection of pyrophosphate release. Analytical biochemistry, 242(1), 84-89. https://doi.org/10.1006/abio.1996.0432
  37. Sabir, J. S., Abo-Aba, S., Bafeel, S., Zari, T. A., Edris, S., Shokry, A. M., ... & Bahieldin, A. (2014). Characterization of ten date palm (Phoenix dactylifera L.) cultivars from Saudi Arabia using AFLP and ISSR markers. Comptes Rendus Biologies, 337(1), 6-18. https://doi.org/10.1016/j.crvi.2013.11.003
  38. Sanger, F., Nicklen, S., & Coulson, A. R. (1977). DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, 74(12), 5463-5467. https://doi.org/10.1073/pnas.74.12.5463
  39. Schauer, N., Zamir, D., & Fernie, A. R. (2005). Metabolic profiling of leaves and fruit of wild species tomato: a survey of the Solanum lycopersicum complex. Journal of Experimental Botany, 56(410), 297-307. https://doi.org/10.1093/jxb/eri057
  40. Schuster, S. C. (2008). Next-generation sequencing transforms today's biology. Nature Methods, 5(1), 16-18. https://doi.org/10.1038/nmeth1156
  41. Sheeja, T. E., Anju, P. R., Shalini, R. S., Siju, S., Dhanya, K., & Krishnamoorthy, B. (2013). RAPD, SCAR and conserved 18S rDNA markers for a red-listed and endemic medicinal plant species, Knema andamanica (Myristicaceae). Physiology and Molecular Biology of Plants, 19(2), 245-250. https://doi.org/10.1007/s12298-013-0166-6
  42. Vos, P., Hogers, R., Bleeker, M., Reijans, M., Lee, T. V. D., Hornes, M., ... & Zabeau, M. (1995). AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research, 23(21), 4407-4414. https://doi.org/10.1093/nar/23.21.4407
  43. Williams, J. G., Kubelik, A. R., Livak, K. J., Rafalski, J. A., & Tingey, S. V. (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research, 18(22), 6531-6535. https://doi.org/10.1093/nar/18.22.6531
  44. Yang, W. J., Nadolska-Orczyk, A., Wood, K. V., Hahn, D. T., Rich, P. J., Wood, A. J., ... & Rhodes, D. (1995). Near-isogenic lines of maize differing for glycinebetaine. Plant Physiology, 107(2), 621-630. https://doi.org/10.1104/pp.107.2.621
  45. Yu, L. R., Stewart, N. A., & Veenstra, T. D. (2010). Proteomics: the deciphering of the functional genome. In Essentials of Genomic and Personalized Medicine (pp. 89-96). Academic Press. https://doi.org/10.1016/B978-0-12-374934-5.00008-8
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Keywords: Biodiversity, DNA Barcode, DNA Taxonomy, PCR, RAPD

How to Cite:
Al-Yassiry, Z. A. N., & Al-Alwani, B. (2022). Estimation of Botanical Diversity by Molecular Marker Methods. Biomedicine and Chemical Sciences, 1(3), 132–137. https://doi.org/10.48112/bcs.v1i3.200
 
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