Abstract

This study aimed for isolation and identification of Klebsiella pneumoniae was an opportunistic pathogen responsible for a wide range of clinical syndromes such as pneumonia in both hospital and community settings. Assessment some immune parameter this study was carried out in Al-Diwaniya Teaching Hospital in Al-Diwaniya Province during the period from December 2018 to February 2019. A total of 272 individuals in both sex: 139 males and 133 females, including 210 sputum and blood specimens were collected from patients with pneumonia and 62 blood specimens were collected from healthy persons as a control group. The result of Microbiological tests of sputum was found 120(57.2%) specimens as Gram negative bacteria. From these specimens found 62(51.7%) have been appeared as positive result for K. pneumoniae and represented a major cause for pneumonia in this study, 37(59.7%) of them were males and 25(40.3%) female. 59(95.16%) of K. pneumoniae isolates have capsule when stained negatively with Indian ink. While, 60 (96.77%) isolates were appeared hypermucoviscosity (HVM) phenotype. The level of interferon gamma (IFN-γ) and toll like receptor (TLR4) concentration was measured by using enzyme-linked immunosorbent assay (ELIZA) and was found that IFN-γ is significantly (p ˂0.05) raised in all age groups of patients in comparison to the healthy control groups. The level of IFN-γ in age group (1-10) years recorded high percentage (293.123) compared with other age groups.. On the other hand, the level of TLR4 concentration was found that is significantly (p˂0.05) raised in all age groups of patients in comparison to the healthy control group. The level of TLR4 in age group (51-60) years recorded high percentage (12.993) compared to other age groups.